In Vivo Cell Culture with Polymer and Helistat |
In Suk Oh, Sun Uk Song, Kyoung Baek Choi, Hyeok Chae Jeong, Kwan Hee Lee |
Polymer와 Helistat를 이용한 여러 가지 세포의 체내 배양 |
오인석, 송순욱, 최경백, 정혁채, 이관희 |
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Abstract |
Purpose : To evaluate a suitable material for in vivo cell therapy and tissue engineering purposes, we have tested biodegradable materials, such as polymer and helistat. Materials and Methods : We injected chondrocytes and fibroblasts with or without TGF-β secreting cells into two types of polymer and helistat, and then injected into mouse peritoneum. The chondrocytes were cultured from patient's knee joint cartilage with degenerative arthritis. TGF-β secreting cells were prepared by calcium phosphate transfection and the TGF-β secretion was measured by ELISA assay. Balb/c mouse was used for in vivo culture. Results : At 6 weeks after injection into helistat, the various cell types with additional TGF-β secreting cells made tissues which were derived from the injected cells. Control cells without TGF-β secreting cells made scanty amount of tissues. Conclusion : Cell therapy or tissue engineering approaches with helistat can be a very useful method for the purpose of tissue regeneration, especially, when they are carried out with additional gene therapeutic approach. |
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